Sakinato Mazidda, Suyadi and Dyah Hikmawati
Abstract
This study aimed to determine the effect of antioxidants onion extract in Ringerââ¬â¢s Dextrose diluent on the quality of Gallus domesticus sperm during storage at room temperature. Onion extract was made using the maceration method with 50% ethanol as a solvent and evaporated with a vacuum rotary evaporator with a temperature of 50 úC. Gallus domesticus sperm was collected using female teaser method. The collected semen was divided into 4 treatments, namely T0 (Ringerââ¬â¢s Dextrose without onion extract), T1 (Ringerââ¬â¢s Dextrose + 0.02 mL of onion extract), T2 (Ringerââ¬â¢s Dextrose + 0.04 mL of onion extract) and T3 (Ringerââ¬â¢s Dextrose + 0.06 mL of onion extract). Evaluation of semen quality during the storage at room temperature including individual motility, viability, and abnormality were observed within 1, 2, 3, and 4 hours. The experimental design used a Completely Randomized Design (CRD), the data analysis used Variance Analysis (ANOVA) and followed by Duncanââ¬â¢s Multiple Range Tests (DMRT). The analysis showed significant differences (P <0.01) for individual motility and (P <0.05) for sperm viability. The best results were shown in T2 (Ringerââ¬â¢s Dextrose + 0.04 mL of onion extract). The sperm quality resulted by T2 treatment could last up to 4 hours at room temperature, with an average of individual motility of 50.83 ñ 3.76b, viability of 76.28 ñ 2.66b and abnormality of 16.87 ñ 1.24a. The addition of 0.04 mL onion extract in Ringerââ¬â¢s Dextrose diluent within 4 hours at room temperature met the quality requirements to be used in Artificial Insemination (AI).