IN-VITRO ASSESMENT OF CHOLIC ACID PERMEABILIZING ABILITY IN COMBINATION WITH ANTIBIOTICS TO COMBAT MICROBIAL RESISTANCE EXHIBITED BY BIOFILM PRODUCING BACTERIAA.I. Sheikh, Shweta Mishra and M. MusaddiqAbstract Biofilm are complex aggregation of bacteria marked by the excretion of a protective and adhesive matrix. They develop almost anywhere in water and solid or solid and gases meet as well as living surfaces, which means they are virtually everywhere, implying their ubiquitous nature. When they form biofilm, bacteria seem to gain super power. In human terms, biofilm spells all kind of big trouble, for instance they fog our contacts, help to rot our teeth and cause a host of diseases from cystic fibrosis and ulcers to colitis and middle ear infection. Microorganism growing in biofilm includes Pseudomonas, Staphylococcus, Streptococcus, Candida, Klebsiella, and Salmonella etc. The nature of biofilm structure and physiological attributes of biofilm organism have found to confer an inherent resistance to antimicrobial agents. Considering the wide variety of microorganism that are able to form biofilms and the mentioned aspects including its environmental, medical, commercial significance the present work was undertaken. Pathogenic organisms were isolated, identified and antibiogram studies were made in presence of usually recommended antibiotics. Screening for biofilm producers was made. With a view to search out for intervention strategies combination of cholic acid was analyzed with antibiotics. The present investigation supports that a link exist between pathogenic organism, biofilm production and drug resistance. It also lead us to conclude the ability of cholic acid in combination with antibiotics to inhibit biofilm producers.
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