Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 14, Issue 3, 2012; Page No.(413-418)

IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF PHYTOPLASMA ASSOCIATED WITH PEACH DISEASE IN WESTERN UTTAR PRADESH

AMIT PRAKASH, ANUPAM SINGH, ASH WANI KUMAR SINGH, SNEHA AND ARCHANA PRAKASH

Abstract

Recently, peach trees were showing symptoms of disease caused by phytoplasma, like leaf reddening, yellowing, leaf rolling symptoms were observed in peach growing areas in western regions of Uttar Pradesh. In nature phytoplasma are transmitted by leafhopper that may have occasional or permanent trophic relationship with their host. Plant pathogenic phytoplasma are wall less, unculturable bacteria of class mollicates with a small genome size, which ranges from 530 to 1350 kilobases. Total genomic DNA was extracted from Different parts of peach tree by CTAB methods and with DNA isolation kit Quiagen (Germany). The Polymerase chain reactions (PCR) were performed using Phytoplasma universal primer pairs. No PCR amplification was observed in the healthy plants. Amplification of phytoplasmal 16S rDNA gene, 16S-23S intergenic spacer region and 5' region of the 23S rRNA gene sequence in PCRs containing phytoplasma universal primer pairs P1/P7, R16F2n/R2 and P1/ Tint and template DNA extracted from diseased peach plant confirmed that the plant were infected by phytoplasma. The Phylogenetic relation to other Phytoplasma was evaluated through iphyclassifier.

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