Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 14, Issue 4, 2012; Page No.(547-552)

ISOLATION OF BACTERIA FROM PERSONAL COMPUTER HARDWARE AND ITS MOLECULAR DETECTION BY 16S RRNA HYPERVARIABLE REGION (V4) SPECIFIC SEQUENCE ANALYSIS

HARIOMSING P. PAWAR1, DEVENDRA P. LINGOJWAR AND VIJAY S. WADHAI

Abstract

Computer hardware may act as a reservoir for microorganisms and may contribute its transfer via user. This research seeks to investigate what kinds of microbes, especially aerobic bacteria that actually can contaminate computer hardware. Bacterial 16S rRNA genes generally contain nine “hypervariable regions” (V1 – V9) that demonstrate considerable sequence diversity among different bacterial species and can be used for species identification. We identified the unknown bacteria isolated from computer hardware by molecular detection and sequence analysis of 16S rRNA hypervariable region V4.The gene sequence obtained was studied in the region from V3-V6. Sequence similarity dendograms were created for hypervariable regions V3-V6. After complete analysis and using bioinformatics tools our investigation demonstrated that the V4 (nucleotide 576-682) contains diverse character and comparatively less distinguish than V3 and V6 for the Bacillus subtilis species molecular identification.

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