Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 14, Issue 4, 2012; Page No.(561-564)

EVALUATION OF ESSENTIAL NUTRIENTS FOR NAVICULA SPS GROWTH BY SUBMERGED FERMENTATIONS

P. BHAGYA RAJ, B. RANJIT AND P. MARY ANUPAMA

Abstract

In vitro cultivation of diatoms to meet the diverse applications is the essence of this work. These microalgae are easy to isolate and cultivate in microbiology laboratories. A minimal medium was used to isolate one diatom sps by its repeated cultivation under nutritional stress. Navicula sps could replicate under the provided conditions and towards the end of 3 weeks a culture enriched in the mentioned sps was obtained. The important nutritional parameter, i.e., silicate and nitrate concentrations required for the isolates optimal growth were determined. Maximum productivity of 50014 diatoms/mL was obtained when sodium silicate at 150?M per L and Nitrate at 250?M per L were provided to the diatoms culture.

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