Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 04, Issue 4, 2002; Page No.(509-513)

ANTIMICROBIAL ACTIVITY OF BALANITES ROXBLIRGIIII ON CERTAIN HUMAN PATHOGENIC MICROORGANISMS

K.M. ELIZABETH

Abstract

The antimicrobial activity of the crude extract of Balanites roxburghii was screened by disc diffusion methods, against certain human pathogenic microorganisms. It was fount that Balanites roxburghii strongly affective against Candida albicans as it showed 54mm zone of inhibition and less effective against S. typhimurium which showed only 16mm zone diameter, while other bacteria were resistant to this herbal extract at 4mg concentration. The minimal inhibitory concentration (MIC) of B. roxburghii towards C. albi cans was 350 ug and minimal bactericidal concentration (MBC) was 2 mg against 1x109 cells, as determined by tube dilution technique. The MIC of this crude extract against S. typhirnurium was 2mg B. roxburghii induced several morphological and biochemical alterations in C albicans at sub inhibitory concentration eventually leading to the death of this organism. But B. roxbuighii was less effective in this regard against S. typhimurium even at a concentration higher than the MIC (4mg/m1). The viable cell counts of C. albicans and S. typhimurium at 350 ug/ml and 4 mg/ml concentrations, showed reduction of 6 and 2 log cycles respectively by 24th hour of incubation. These results clearly indicate that B. roxburghii crude extract has antimicrobial activity against C. albi cans (fungicidal) and S. typhimurium (bacteriostatic). But it was ineffective against other bacterial pathogens at the concentrations used.

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