Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 07, Issue 2, 2005; Page No.(191-194)

IN VITRO REGENERATIVE POTENTIALITY OF ANNONA RETICULATA L. AND ANNONA SQUAMOSA L.

ROXANA AHMED, USHA RANI DAS. AND SYED HADIUZZAM

Abstract

Shoot tips and nodal segments of field grown mature plant and shoot tips and hypacotyLs of axenic seedling of Annone reticulate and Annona square.) were tested for in vitro regeneration. Nodal segments of mature plant of A. reticulate were found more responsive as compared to A. squaws». A. sqoenrosa produced a good number of multiple shoots from hypocotyls of seedling. The highest number of microshoots from nodal segment of mature plant of A. reticule:a was observed in MS medium containing 2.0 mig/L BAI40.5 rng/L Non 2.5 mg/L IBA whereas maximum number of inicroshoots per hypocotyl of seedling of A. squawur was obtained in MS medium suppleinented with 2.0 mg/L BAP n0.) mg/L NAA • 0.5 mg/L Kn. During the experimental period leaf abscission and shoot death were found in all the regenerated shoots irrespective of the media and explant used. To control leaf abscission and shoot death silver nitrate added medium with micro filter membrane as closure was found to be the mcst effective when the treatment was given to A. squaw., Root induction of A. reticulate was found only on 30.0 mg/I. IBA containing half MS medium. In case of A. squaw. half MS media supplemented with 25.0 mg/ I. IBA was found the best for root induction. 1801202012

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