Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 07, Issue 3, 2005; Page No.(387-390)

COMPARISON OF FOREST WASTE DEGRADATION UNDER SOLID STATE FERMENTATION AND SUBMERGED FERMENTATION BY COCULTURE OF BACILLUS LICHENIFORMIS AND B. COAGULANS

N. SHARMA AND K.L. BANSAL

Abstract

Bacillus lichen iformis and B. coagulans were found to be the potent producers of cellulase and xylanase isolated from degrading wood and soil. Both these isolates showed better growth under coculture conditions. The waste of forest species Pin us roxburghii, Cedfus deodara, Quercus leucotricophora and Robinia pseudocacia were selected as substrates for biodegradation studies depending upon their wide abundance, local availability and industrial use. The wood waste was subjected to various physicochemical pretreatments for enhancing the degradation. Their biodegradation was compared under submerged fermentation (SmF) and solid state fermentation (SSF) conditions. SSF using tap water was found to be superior over SmF showing maximum per cent increase in BI of 174.90 in dil FI2SO4 pretreated Robinia. Replacing tap water with modified basal salt medium in the degradation medium has further increased SSF of biomass. Biodegradation scaled up considerably when tap water was replaced with modified BSM touching the record level of 2350 per cent increase in dil HNO, treated Robinia as compared to its SSF with tap water.

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