Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 07, Issue 3, 2005; Page No.(421-426)

ISOLATION OF HALOALKALOPHILIC WEEDICIDE TOLERANT RHIZOBILIM FROM NODULES OF CAJANLIS CA JAN

R.C. SHENDE AND M.B. PATIL

Abstract

Two Rhizobium strains were isolated from the nodules of Cajanus cajan plants grown in different soil conditions. The normal (N) strain was isolated from soil with normal electrical conductivity and haloalkalophilic (H) strain was isolated from nodules of plant grown in saline soil with high electrical conductivity. The optimum pH and salt requirement of growth of N strain was 6.0 and 100 mM respectively, whereas the H strain had optimum pH of 8.0 and salt requirement of 300 mM respectively. The weedicide tolerance (L1D,o) of N strain towards g/yphosate was 4% ai as compared to the 6% ai for H strain. Both N and H strains nodulated the C. cajan plants at their specific optimum conditions and even in presence of glyphosate.

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