Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

ISOLATION OF TEXTILE DYES DECOLORIZING MICROBES AND THEIR OPTIMIZATION OF CULTURE CONDITIONS

S.B. GONDALIYA AND S.J. PATHAK

Abstract

Efforts were made to isolates indigenous microbes from the textile dye contaminated site. A total of 35 strains of organisms were isolated based on colonies and morphological charactreristics and were primary screened for their ability to decolourized the textile dye Remazol magenta HB under static culture conditions in presence of three different nutrients in minimal salt medium. Primary screened out 10 isolates were further screened for decolourisation ability against sixty different commercial textile dyes and the most potent six isolates have been further studied for their specific requirements to obtain maximum declourization capability in cultured condition. Results of dyes acted by the isolates were observed that the 'so-32 strain has completely decolourized maximum number of dyes i.e. thirty one dyes followed by Iso-26 showed twenty nine days and other four stains acted on more than twenty days. Most potential of these six strains Bio-1 to Bio-6, were subjected for optimization of influences of various nutrient ingredients supplements such as carbon sources, nitrogen sources and nutrient and/or growth factors. Results were found that in presence of the yeast extract, beef extract, multivitamin solution and riboflavin individual in minimal salt media showed maximum near about to 100% decolourized within 24 hours of incubation at 30°C temperature under static condition. In case of inorganic nitrogen sources conclusive results were seen with ammonium sulfate, di-ammonium hydrogen phosphate and ammonium chloride, whereas, nitrate and nitrite salts of ammonia, the process of decolourization was very less (10% to 46%), suggesting that NO3- or NO2- might have been adverse effects on decolourisation. Among the various sugars tested at varies concentrations for their effect on dye decolourization by the six isolates glucose, sucrose, lactose, and mannitol were showed maximum at about 0.5% concentration except Bio-6, which required 1.0% to get maximum decolourisation. Textile dveincr And nrintino- industries have very