Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 07, Issue 3, 2005; Page No.(577-582)

SEED MYCOULORA AND STORAGE EFFECT IN THE GERMINATION OF FRENCH BEAN (PHASEOUTS VULGARIS LINN.)

A. BHATTACHARJEE AND M.S. DKHAR

Abstract

Local variety of French bean (Phaseolus vulgaris Linn.) viz Meghalaya has been selected for the present study. Of the three methods used for the isolation of fungal species, blotter method proved to be the most effective followed by agar method. The dilution plate method was least effective in harbouring the fungal species vis-avis the two methods. A total of 26, 28 and 28 fungal species were isolated by dilution plate, agar and blotter methods respectively. The fungal species such as Aspergillus niger, Cladosporium cladosporioides, Colletotrichum lindemuthianurn, Fusarium oxysporum, Pythium intermedium and Trichoderma viride were found to be present in all the seasons of the year. The storage of bean seeds in different containers recorded maxmum number of fungal species in rainy season followed by summer and minimum was observed in winter season. The storage of seeds in earthen pots had a negative effect on the overall rate of germination. The impact of storage on seed was slightly better in bamboo basket followed by gunny bag. Seeds stored in iron bins exhibited a very low level of mortality. With increase in storage period, the viability of the seeds decreased and the germinability reduced. Significant variation in percentage fungal infection was observed between gunny bag and earthen pot and between earthen pot and iron bin at 0.05 level.

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