Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 08, Issue 2, 2006; Page No.(229-233)

ISOLATION OF BACTERIOPHAGES SPECIFIC TO PSEUDOMONAS AEUROGINOSA

P. DHEVAGI*, V. BHARATH KUMAR, M.A. SHIBU AND M. RABEETH

Abstract

Bacteriophages specific to Pseudomonas aeuroginosa have been demonstrated in albino mice under invitro condition. Pseudoinonas aeuroginosa was isolated from infected person and characterised. After characterization bacteriophages specific to Pseudomonas aeuroginosa was isolated from phage enriched sewage sample. Minimal lethal dose of Pseudonionas aeuroginosa was found to be 3 x 103 CFU/ml. Phages specific to Pseudomonas aeuroginosa were injected to mice after injecting with the pathogen. Results showed that mice treated with lower concentration of lysate showed the symptoms of lethargy. As the phage dose increased from 200 to 800x10" PFU/ml complete recovery of the animal same as control was observed.

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