Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 08, Issue 2, 2006; Page No.(249-251)

TRIGGERING OF CELLULASE PROTEIN PRODUCTION USING CELLULOSE WITH LACTOSE BY TRICHODERMA REESEI

R.MUTHUVELAYUDHAM, S. DEIVEEGAN & T.VIRUTHAGIRI

Abstract

The production of cellulose protein in batch culture was studied using Trichoderma reesei on the mixtures of cellulose and lactose. The work was carried out for the various mixtures of lactose and cellulose for the period of seven days. The cellulose activity, amount of substrate and amount of protein were estimated by standard methods. In mixed substrates a diauxic pattern of utilization of the two carbon sources were observed. The lactose was utilized first to support growth, followed by cellulose to induce the cellulose protein production and provide and additional carbon source for cellular metabolism. The kinetic study were also made for both growth and product formation.

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