BACTERIAL DECOLOURIZATION AND BIOREMEDIATION OF AN ANAEROBICALLY DIGESTED MOLASSES SPENT WASHN. SIVAKUMAR, V. SARAVANAN, AND S. BALAKUMARAbstract At the present investigation were known to be capable of dealing with the reduction of COD and colour using native bacterial cultures suchas Bacillus megatherium SW3 and Bacillus subtilis SW,. Bacterial enrichment cultures were set up to obtain isolates capable of DMSW decolourization and bioremediation .Two bacterial cultures SW3and SW, were obtained using effluent sample from a Trichy Distillery plant. Both grown well in 7.5% (v/v) diluted digested spent wash supplemented with glucose and urea as a readily available carbon and nitrogen source. Optimum condition for growth and DMSW decolourization were at pH 7.2, temperature 37"C, 5% (w/v) of glucose, and 2% (w/v) urea in a minimal salt medium. The maximum decolourization (51%, 52.5% and 57%) and COD reduction (53%, 54.5% and 59%) was found after 5 days of incubation under the optimized condition were achieved for cultures SW3, SW8and consortia (SW3+ SW) respectively.
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