Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 8, Issue 3, 2006; Page No.(461-464)

FUNGITOXIC AND CYTOTOXIC ACTIVITY OF A NOVEL COMPOUND 1,2-BENZENEDICARBOXYLIC ACID, DIISOOCTYL ESTER OF PLUMBAGO ZEYLANICA LINN

MD. SHAFIQUR RAHMAN AND M.N. ANWAR

Abstract

1,2-Benzenedicarboxylic acid, diisooctyl ester (diisooctyl phthalate) isolated from roots of Plumbago zeylanica was screened for its an tifungal activity against six phytopathogenic fungi viz., Alternaria alternata, Botryodiplodia theobromae, Curvularia lunata, Fusarium equiseti, Macrophomina phaseolina and Colktotrichum corchori. The antifungal activity of the compound was evaluated using poisoned food and broth macrodilution methods. The diisooctyl phthalate (200pg/mL and 100 pg/mL medium) inhibited the radial mycelial growth of all the test fungi to varied degrees. The lowest MIC (10 pg/mL) and MFC (20 pg/mL) values were recorded against M. phaseolina, and M. phaseolina and B. theobromae respectively. Cytotoxic activity (LCro) of the compound has been determined on newborn brine shrimp (Arterma salina). LC50of the compound was recorded as 8.69 1.1g/mL against newborn brine shrimp.

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