Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 8, Issue 3, 2006; Page No.(601-604)

ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF AMYLASE PRODUCING MICROORGANISMS FROM BAY OF BENGAL, VISAKHAPATANAM, ANDHRA PRADESH

K. M. ELIZABETH, CH.V.N.CHAITANYA AND P.SURIBABU

Abstract

Bacterial amylases are very important with several commercial uses. Two bacteria were isolated, identified as Bacillus species (Si& M1) from marine waters of "Bay of Bengal", Visakhapatnam. The amylase production by these two microorganisms was studied at various pH, temperatures and in different media. The effect of different temperatures on enzyme activity of these two bacterial isolates was also carried out. There was highest production of amylase ( 3066 ug / mL) by Bacillus species - when cultured in Luria Bertain broth for 24 h at pH. 8, indicating that the ingredients of this medium can induce amylase production than the other two media used such as amylase production medium and nutrient broth. The enzyme activity was highest at 45 °C and 35°C in Si & Ml strains respectively. These results indicate that these two marine microorganisms can be used for commercial extraction of amylase enzyme for industrial purpose.

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