Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 8, Issue 3, 2006; Page No.(641-642)

IDENTIFICATION OF INDIAN CASSAVA MOSAIC VIRUS USING MOLECULAR TECHNIQUES

A. MARY VIOLET CHRISTY

Abstract

Cassava Niosaic 1case (CMD). caused by indian cassava mosaic a mosaic virus in India is a major cc7,st-ain for cassava cultivation. The success of ma n.ement Mies on the use of virus free panting material coupled with sensitive detection technicius. Meristem culture pnwed to be an iml.-ortant tedmique for virus elimination. In the present study meristem culture samples of different casswa varieties were indexed for the absence or presence of the virus. Seven varieties of cassava were established M the present study. While performing IC. SC and PC-PCR meristem cultured samples of H-165 showed amplification together with the ICN IV infected cassava field samples. But meristem cultured samples of Sree prakaskhand H-226 showed no amplification indicating the absence of the virus.

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