Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 8, Issue 4, 2006; Page No.(783-784)

MOLECULAR VARIABILITY IN THE MOSAIC DISEASE FREE ACCESSIONS OF MANI TIOT ESCULENTA CRANTZ.

RINTU T.VARGHESE, A. PENITTA AND SANTHA V. PILLAP

Abstract

Cassava (Manihot esculenta Crantz) is one of the most important food crops of the tropics along with rice, maize and sugarcane. It ranks as the fourth source of carbohydrates in the tropics (FAO, 1995). Cassava is also an industrial crop for starch, flour and animal feed. Cassava mosaic disease caused by a gemni virus is a serious problem in cassava and selection (or) breeding for disease free varieties is very important. In the present study some of the cassava mosaic disease free varieties were analyzed for DNA polymorphism using RDPD analysis. Various markers for morphological and agronomical traits are traditionally used for divergence and characterization studies of cassava cultivars. To find the molecular variability available in the cassava mosaic disease free accessions of cassava using DNA Polymorphism and document the data. The RAPD of the extracted DNA using 3 primers showed polymorphism in 9 accessions out of the 12 accessions studied. 21 different polymorphism bands were obtained. Dendrogram showed that, the similarity varied between 32.84% and 100%. From the study, it can be concluded that RAPD analysis is an additional tool to identify molecular variability and genetic relatedness in cassava.

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