Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 8, Issue 4, 2006; Page No.(785-789)

ISOLATION AND SCREENING OF YEASTS FOR ETHANOL AND XYLITOL PRODUCTION FROM D-XYLOSE

CH. PAVANA JYOTHI, R. SREENIVAS RAO AND L. VENKATESWAR RAO

Abstract

Natural habitats of yeasts were examined for the presence of strains, which were able to produce xylitol and ethanol from D-xylose. Six different MTCC strains of Candida sp. (4), Pachysolen sp. (1) and Pichia sp. (1) have also been examined for the production of ethanol and xylitol from D-xylose. The isolated strains showed the ability to assimilate D-xylose, but the production of ethanol and xylitol was very low, whereas the six MTCC strains fermented D-xylose. Candida boidinii produced ethanol, Candida tropicalis, Candida parapsitosis, Pichia jadinii produced xylitol, Candida intermedia and Pachysolen tannophiles produced both ethanol and xylitol from D-xylose. The yeast Candida intermedia MTCC-1404 has been selected for further study, because it has produced more amount of ethanol (70%) and xylitol (11.1%). The effect of different carbon sources for the production of ethanol from D-xylose has been studied using Candida intermedia. In the presence of maltose, the ethanol yield increased to 9% and with sucrose, ethanol yield increased to 5%.

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