Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 9, Issue 1, 2007; Page No.(31-36)

ASSESSMENT OF GENETIC DIVERSITY OF COTTON CULTI VAR') (GOSSITILTM SPP,) BY ISSR AND MICROSATELLITE MARKERS

A.B. DONGRE, MANOJ BHANDARKAR, RAVI SAWANT, LALIT KHARBIKAR AND ARCHANA GAJBHIYE

Abstract

In the present study twenty-five selected germplasm belonging to G. hirsutum were subjected to DX A based microsa tell ite and ISSR analysis, with the major objective of characterization and assessment of genetic diversity at DNA level and to study the extent of genetic variation present among those accessions. The two-marker system rnicrosatellite and ISSR markers were used to generate DNA profile of 25 genotypes of cotton, and each amplified DNA marker fragment was recorded. The results of cluster analysis indicated not only the separation of genotypes of the species (G, hirsuttsm) into separate groups; but also the separation of the genotypes among each group. The similarity coefficient based or. ISSR and rricrosatellite markers analyses, ranged from 0.57 to 0.10 and 032 to 0_92, respectively, suggesting a considerable genetic variation between the cotton species studied.

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