Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 9, Issue 1, 2007; Page No.(99-106)

BIOCONVERSION OF CELLULOSE TO ETHANOL USING MUTANT STRAIN OF FLISARILIM OXYSPORLIM

V. SARAVANAN, P. NAGARAJAN, T. KARUNANIDHI AND T. VIRUTHAGIRI

Abstract

The present investigation deals with development of mutant strain of fungi for studying enhanced 1.‘n.Iductivity of ethanol from cellulose in batch fermentation. The filamentous fungus, cellulose utilizing Fusaritim oxtisporum (NITCC.Chandio-arh) is known for its ability to produce ethanol by simultaneous saocharification and fermentation of cellulose The parental culture of Fuzwriunt olysTonon was mutated using UV light. The influence of parameters, temperature, pH, initial substrate concentration and production of ethanol using five mutated strains of fungi under optimized conditions of temperature, pH.. and initial substrate concentration was found. Optimum temperature, inoculums and pH for mutant strain 2 were found to be30`C, and 4.5 respectively. The mutant strain 2 -was found to be efficient to ferment at least 7% i$ v) concentration of substrate. The maximum production of ethanol of 15.02g/L was obtained using mutant strain 4 efftted bv '5 minutes of CV rays exi_losure. The kinetics of ethanol production using mutant strain 4 of fungi in batch fermentation under optimized conditions was carried out. The experimental data weiv used to simulate the various models to describe the kinetics of ethanol pitIduction Monod model was found to fit the data for batch growth kinetics and Leudeking-piret mt-kiel was used for both product formation and substrate consumption kinetics. The kinetic parameters of all the models are used to stimulate the profile of biomat--,s and product duriikg the entire course of fermentation.

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