Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 9, Issue 2, 2007; Page No.(243-244)

ENHANCEMENT OF HOST SPECTRUM ACTIVITY OF B. THURINGIENSIS AND B. SPHAERICLIS BY CONJUGATION

SANJAYKUMAR S. INGLE

Abstract

Bacillus thuringiensis var. israelensis (Bti) and Bacillus sphaericus (Bs) are the potent and safe mosquito larvicidal bacteria having different properties and host specificities. Bti is more toxic to Aedes and Culex mosquito larvae where as Bs is more toxic to Culex and Anopheles mosquito larvae and not against Aedes. Host spectrum activity of these entomopathogens was increased by cell mating among Bti and Bs. Maximum transcipients were isolated from mating mixture at 4 h of incubation period. The LC5os of transcipients TC1, TC2 and TC3 toxin were found to be 0.026, 0.04 and 0.03 jAg/m1 respectively. ICPs of transcipients TC1, TC2 and TC3 showed immunoreactivity with polyclonal antibodies raised against total solubilized crystal protein of Bti and Bs.

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