PRODUCTION OF POLY B HYDROXYBUTYRATE BY ALCALIGENES EUTROPHUS AND BACILLUS MEGATERIUM USING MOLLASESS. ASOKAN AND C. SUSITHRAAbstract In this present study, two bacterial species were isolated from the municipal waste disposable site located at, Peravurani, Thanjavur District of Tamil Nadu. By using these isolates an attempt was made to produce PHB by using molasses as a substrate. Culture conditions like pH have been optimized with different carbon and nitrogen sources in different concentrations. Alcaligenes eutrophus showed a higher percentage of PHB production (40%) and Bacillus megaterium was yielded a moderate results. Synthetic fermentative medium was used as a control. Optimized pH was observed as 7. Other parameters like carbon (maltose) and nitrogen (ammonium chloride) supplementation were also evaluated and the highest (0.524 g/L) productivity was observed in maltose supplemented medium when comparing the slightly lower level in case of sucrose supplemented medium (0.493 g/L).
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