Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 7 Issue 4, 2005; Page No.(759 -762)

IN VITRO EVALUATION OF ANTIMICROBIAL PROPERTIES OF STEM WITH LEAF EXTRACT OF CURANGA AMARA JUSS AGAINST HUMAN PATHOGENIC MICROORGANISMS

A. KAR, UK. GOGOI, B. RABFIA AND H.K. GOGOI

Abstract

The crude stem with leaf extract of Curanga amara Juss. was screened in vitro against five human pathogenic microorganisms by Agar well diffusion method. Five different organic solvents were used to prepare stem with leaf extract of the plant and their efficacy was tested. The crude acetone extract was found most effective against the five rest organisms e.g. Escherichia coli, Bacillus subtilis, Klebsiella sp., Staphylococcus aureus and Candida albica,Is respectively. A comparative study against commercially available antibiotics were carried out by dis*. diffusion method where it was found that B.subtilis, E. coli and Klebsiella sp. are resistant to Penicillin G and Ampicillin. No other antibiotic except Albendazole was found active against C. albicans. But the crude acetone extract of C. arnara was effective against all the test organisms. The minimal inhibitory concentration (MIC) of C. amara extract towards E.coli, B.subtilis and S.aureus was Img against 1 x 108 cells as determined by tube dilution techniques. The MIC of acetone extract of C. amara for Klebsiella sp was 1.5 mg and towards C. albicans was 2 mg against 1 x 108 cells. Present finding suggests the usefulness of crude acetone extract of C. amara against human pathogenic bacteria and fungi.

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