Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 10, Issue 2, 2008; Page No.(283-290 )

PHYSICO-CHEMICAL OPTIMIZATION OF TANNASE PRODUCTION BY KLEBSIELLA PNELIMONIAE MTCC 7162 USING FRACTIONAL FACTORIAL STATISTICAL METHODS

J. ARAVIND, S. KA RTHIKEYAN AND KUNTHALA JAYARAMAN

Abstract

Tannase an enzyme capable of hydrolyzing tannins was produced by one of our bacterial isolates from tannery wastes and later identified as Klebsiella pneumonia(' MTCC 7162. Five nutritional parameters (tannic acid, NaNO3' K2HPO4, KC1 and MgS04'7H20) and three physical parameters (pH, temperature and agitation) were screened for their effect on increased production of tannase using modeling and design software. Theoretical experimentations of batch mode were considered for fermentor run which resulted in a three fold increase in the tannase production (41.5 U/mL) than the batch studies (14 U/mL) and over all 10 fold increase than non-optimized studies (4 U/mL).

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