RAPID CLONAL MULTIPLICATION OF THE MEDICINAL LEGUME, CAESALRINIA BONDLIC (L.) ROXBP. KANNAN, K. RAJA LAKHM, G. GRACE LYDIAL PUSHPALATHA AND G.C. ABRAHAMAbstract Cars & pin ia bonduc (L.) Roxb, the over exploited and fast depleting medicinal straggler is regenerated from immature cotyledon and hypocotyl explants through direct organogenesis without an intervening callus phase. Adventitious shoots can be raised from cotyledon and hypocotyls on medium augmented with select concentrations of cytokinins and auxins. Supernumerary shoot production can be achieved in hypocotyl segments on medium with 0.1-2.5 mg/L BA while adventitious shoots can be induced on excised cotyledons on MS medium supplemented with 0.1-3.0 mg/1 6-benzyladenine (BA). Higher frequency shoot development (85 %) with an average of 10.0 ± 0.08 shoots sprouted out from deembryonated excised cotyledons in media fortified with 2.0 mg/L BA. The redifferentiatecl shoots, irrespective of its origin in different explants and cultural history, could be successfully rooted on half strength MS medium supplemented with 0.5mg/L indole-3-butyric acid (IBA). Whole plants recovered effectively form this subculture sequence were established successfully in soil to register 70 % survival in the field.
Enter your contact information below to receive full paper.
|