FERMENTATION OF MUNICIPAL SOLID WASTES BY BACTERIAL ISOLATES FOR PRODUCTION OF RAW PROTEIN DEGRADING PROTEASESABUL KALAM AZAD, AKHIKUN NAHAR, MD. MAHBUB HASAN, KAMRUL ISLAM, MD. FAISAL AZIM, MD. SADDAM HOSSAIN, MOHAMMAD REJAUR RAHMAN, RAVI KANT OJHA, G.M. SHAMIM MAHMUD AND RAFIDIN KAYESAbstract To produce extracellular bacterial proteases using municipal solid wastes (MSW), we isolated three proteolytic bacterial isolates from MSW and identified them as Bacillus sp., Pseudomonas sp. and Serratia marcescens. Optimum temperature and pH for maximum protease production by Bacillus and Pseudomonas isolates were 37 oC and 7.0, respectively and those by S. marcescens were 30 oC and 8.0, correspondingly. With proteinous and cellulosic MSW, cultivation period for optimum protease production by Bacillus and Pseudomonas isolates was 60 h and that by S. marcescens was 48 h, however, with a basal media, it for Bacillus, Pseudomonas and S. marcescens was 48, 42 and 24 h, respectively. Crude proteases from the three isolates were stable at pH 6.0-8.0 and at temperatures 25-45 °C and were either cysteine and/or serine types. Proteases from Bacillus, Pseudomonas and S. marcescens degraded about 20, 45 and 50% dry weight of the chicken meat, respectively after 48 h of treatment.
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