BIOSYNTHESIS OF ALKALINE PHOSPHATASE BY NON-SYMBIOTIC DIAZOTROPHSSOMA PAL SAHA,ANINDITA BHATTACHARYA, SAGNIKA GHOSH, SUBHASIS GHOSH, SOURAV GHOSH, HUMA SHAZ AND SWAPAN BHATTACHARYYAAbstract A large number of diazotrophic organisms have the ability to solubilize the immobilized phosphate. These microorganisms can be used as efficient biofertilizers to supply sufficient amount of nitrogen and phosphorus to the plants. Following enrichment and dilution plating on nitrogen-free Stockdale tricalcium phosphate medium such organisms were isolated from the soil. Among a subsample of 15 isolates, 14 were gram negative and 80% of them were coccoid. An efficient phosphate soulubilizng isolate, strain UT01 had been presumptively identified as Azotobacter sp. The cultural condition for optimum biosynthesis of phosphatase by this strain under laboratory condition was determined. In the said medium with 0.5% (w/v) phosphate, the strain secreted 16.5 units of enzyme/ml of culture. Medium with lower concentration of phosphate 0.3% (w/v), pH 8.0 and incubation temperature 35oC, appeared as growth promoting and this condition accelerated enzyme secretion by 43%. Furthermore, the strain synthesized phosphatase enzyme, which showed almost same activity at pH ranging from 8.0 to 9.0.
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