Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 15, Issue 4, 2013; Page No.(717-723)

COMPARATIVE STUDY FOR BIOSYNTHESIS OF SILVER NANOPARTICLES FROM PSELIDOMONAS PUTIDA NCIM 2650

V. THAMILSELVI AND KV. RADHA

Abstract

This study reported on the extracellular synthesis of silver nanoparticles from bacterial strain Pseudomonas putida NCIM 2650. The silver resistant ability was studied by incubating bacterial strains of Pseudomottas putida NCIM 2650, Bacillus subtilis NCIM 2655 and Lactobacillus acidophilus NCIM 2903 on the Agar plate supplemented with silver nitrate with different concentrations. The tolerant level of silver by those bacteria was found to be 1mM concentration. The silver nanoparticles synthesized from both Pseudomonas putida NCIM 2650 and its silver resistant strain was compared and characterized by UV spectroscopy and SEM. The maximum UV absorbance peak was observed at 420nm. The size morphology of the Pseudomonas putida NCIM 2650 was analysed from SEM Micrograph shows agglomerated and the resistant strain was polydispersed and found to have sizes ranged between 38 and 118nm. The parameters pH and temperature for optimum synthesis was found to be at 6 and 60 °C respectively. The optimized silver nanoparticle was subjected to cloud point extraction using non ionic surfactant. The extracted sample of silver nanoparticles showed the enhanced effect of antimicrobial activity against gram positive bacteria, gram negative bacteria and fungi.

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