Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

ASSESSMENT OF DIFFERENT PARTS OF ANNONA SQUAMOSAL. FOR THEIR ANTIMICROBIAL ACTIVITY AGAINST PATHOGEN ISOLATES FROM RAIPUR REGION

RINU KHAN AND A.K. GUPTA

Abstract

Annona squamosaL. member of the Annonaceae family, also known as custard apple, is commonly found in deciduous forests and is cultivated in wild in various parts of India. In this investigation, antimicrobial effect of root, seed and leaves extracts of Annona squamosaL. on clinical isolates of three Gram negative and one Gram positive pathogens from Raipur Region were studied, using paper disc method. Gram negative and positive microorganisms studied were Escherichia coli, Klebsiella pneumonia,Pseudomonas aeruginosaand Staphylococcus aureus. For examination of antimicrobial properties of A. squamosaon pathogenic microorganisms, disc diffusion method using different parts of the plant extracted with four different solvents namely petroleum ether, acetone, ethyl alcohol and water were used. Among all, the root of Annona squamosawas found to be more effective as compared from seed and leaf. The zone of inhibition was found to be more pronounced in Pseudomonas aeruginosa (7.66 ± 0.33) for the acetone extraction of root of A. squamosafollowed by Escherichia coli(6.66 ± 1.20), S. aureus(4.66 ± 0.88) and Klebsiella pneumonia. Seeds of A. squamosaalso showed similar pattern of inhibitory effects on tested organism lesser than that of root. The leaf of this plant showed lower antimicrobial potential compared from root and seed. Aqueous extract did not express satisfactory result against clinical isolates. The activity index of root of A. squamosawas found to be greater as compared to the activity index of the seed and leaf. The phytochemical analysis revealed that the plant is rich in chemicals such as alkaloids, flavonoids, saponins, sterols, tannins and terpenoids. The results suggested that acetone could be effectively used for the extraction of phyto compounds from A. squamosa. Similarly the root of the plant could be used as important source for antimicrobial property against clinical isolates.