Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 15, Issue 4, 2013; Page No.( 799-804)

A NOVEL METHOD OF USING BIOSENSOR WITH NANOPARTICLES FOR THE DETECTION OF PATHOGENIC BACTERIA IN FOOD

V. MANONMANI , A.VIMALA JULIET AND K. PRAVEEN KUMAR

Abstract

A key step in food borne pathogens control is to effectively detect pathogens along food production and processing line in a timely manner. Conventional methods range from color based to fluorescence based, from immunology based to PCR based tests. These approaches being potential most of them are time consuming and difficult. Nanoparticles are usually used either as labels or separation aids or both in pathogen detection procedures because of unique optical, electrical or magnetic properties. In this paper, a new model of biosensor was proposed which offers several benefits over existing methodologies. Biosensors emerged with nanotechnology approaches, holds great challenge for addressing the needs of medical diagnostic systems. In this study, we developed a Nano Biosensor which consists of various stages such as synthesis of Magnetic nanoparticles, it’s surface modification with chitosan, characterization using UV-Visible Spectroscopy and TEM, dye conjugation (Rhodamine and congo red), and finally the detection of bacteria using fluorescence spectroscopy. The basic concept behind the work is that the chitosan, a biodegradable polymer from amine group is used for separation of pathogenic bacteria which, specifically binds onto the surface of bacterial cell wall due to attraction of electrostatic charges and protein-receptor interactions. Thus the obtained chitosan magnetite complex was recovered from reaction mixture by using an external magnet. And then, the surface modified bacteria’s were separated and incubated at 370C for few minutes. This test sample is being conjugated finally with 10 μl of rhodamine dye which will emit light upon input source of light. Similarly, test sample being conjugated with congo red dye also. Bacterial presence was being detected and measured in terms of fluorescence intensity using biosensor scheme.

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