DECOLOURIZATION OF TEXTILE AZO DYES BY LACCASE PRODUCING BACILLUS SPP. ISOLATED FROM SOILVIRAJ KRISHNA MISHRA AND HIMANI SHARMAAbstract Twenty five bacterial isolates exhibiting laccase activity screened on the basis of 0.1% guaiacol utilization from field soil. LB media containing Cu2+ was used for isolation screening. Out of twenty five only three isolates were selected on basis of dye decolorization activity and identified as Bacillus spp. on basis of biochemical characterization. Selected isolates D1023, D1032 and D1033 were transferred to 2×SG medium to increase and fasten spore production. Selected isolates could grow from temperatures ranging from 30°C to 55°C and showed optimum growth temperature and pH at 37°C and 7.0, respectively. Maximum growth was observed using fructose as carbon source and at 2.6% concentration of nutrient broth. Spore laccase of isolates D1023, D1032, D1033 exhibited the excellent potential to decolorize different azo dyes: Amido Black, Orange G, Methyl Orange, Congo Red and Solochrome Black at a concentration of 100 mg/L maximum up to 83.43%, 91.42%, 78.14%, 72.42% and 71.31% respectively during a time period of 4 days at 37 °C (pH 7) during static conditions, which suggests the potential application of spore laccase in dyestuff treatment.
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