GC-MS ANALYSIS OF PIMPINELLA TIRUPATIENSIS, BAL. & SUBR. TUBER EXTRACT: AN ENDEMIC PLANT TO TIRUMALA HILLS OF EASTERN GHATS, INDIAP. UMA MAHESWARI, S. K. M. BASHA, M.RAMBABU AND C.V. NARASIMHA MURTHYAbstract Pimpinella tirupatiensis Bal. & Subr. (Family Apiaceae; local name, kondakothimera) is a rare and endemic medicinal plant and restricted to the Seshachalam hills of the Eastern Ghats, India (Balakrishnan and Subramanyam 1960, Nair and Sastry 1998). The whole plant of P. tirupatiensis is used to treat cough, stomach, liver problems, asthma, ulcer and tooth ache (Reddy and Reddy, 1998, Madhava Chetty et al., 2008). This plant root extract is also used to treat skin disease and also as antimicrobial agent (Jeevan Ram et al. 2004, Bakshu and Venkata Raju 2004). The aim of the study is to investigate the phytochemical analysis of alcoholic tuber extract of Pimpinella tirupatiensis Bal. & Subr by qualitative and GC-MS methods. The qualitative studies of the tuber revealed the presence of Tannins, Steroids, Alkaloids, Glycosides, Flavonoids and Phenolic compounds. In the GC-MS analysis, 24 bioactive phytochemical compounds are identified in the alcoholic extract. Among them two important photochemical compounds Germacrene D (4.35%) & and a-Humulene(1.45.6%) are present in rich amount. Germacrene are a class of volatile organic hydrocarbons, specifically, sesquiterpenesy produced in certain plant species and they are known for their antimicrobial and insecticidal properties, though they also play a role as insect pheromones. P-Cymen is a volatile oil which found to have antioxidant property.
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