Pollution Research Paper

Vol 33, Issue 02, 2014; Page No.(473-480)

A STUDY ON ANTHRACENE DEGRADING POTENTIAL OF MARINE BACTERIAL SPS AND THE GENE RESPONSIBLE FOR THE CATABOLISM

SWAATHY, S., KAVITHA, V., SAHAYA PRAVIN, A., SEKARAN. G., MANDAL, A.B., AND GNANAMANI, A.

Abstract

Anthracene, a tricyclic aromatic hydrocarbon causes many problems associated with health and environmental impact and serves as a signature compound to detect PAH contamination. Destruction of anthracene molecule in aqueous media or in soil phase without generation of toxic intermediates is a challenging task and the existing physical and chemical treatment facilities are inefficient in the destruction or degradation of anthracene. Microbial destruction, the option available has limitations with bacterial species. The present study emphasizes, effective degradation of anthracene by the marine isolates of Bacillus genus. The five marine isolates having different morphological pattern but differ at species level irrespectively showed tolerance towards anthracene at higher concentration (>500 mg/L) and degrade >95% at concentration < 500 mg/L in aqueous medium with in 36 days. The end product of degradation was identified as Octacosane (a linear chain aliphatic molecule) based on FT-IR, and GC-MS spectral analyses. When these species subjected to gene amplifications studies using the primers designed for C23O gene, it is interesting to observe that all the five species displayed the the amplified product at 1.3kb. The study concludes that, marine bacillus sps able to destruct anthracene effectively through its C23O gene.

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