Ecology, Environment and Conservation Paper

EVALUATION OF EFFECTIVE LOCAL RHIZOBIAL ISOLATES FOR NITROGEN FIXATION RESPONSIVENESS VARIETY OF SOYBEAN (GLYCINE MAX)

R. Naveen Kumar, R. Subhash Reddy, R. Sudhkar and B.H. Sarvani

Abstract

In the present investigation, a total of fourteen rhizobial isolates were obtained from the Soybean root nodules and rhizospheric zones of different parts of Adilabad district. The bacterial isolates were authenticated as Bradyrhizobium on the basis of cultural, morphological, biochemical and nodulation tests. These rhizobial isolates were screened for plant growth promoting attributes such as phosphate solubilization, IAA and siderophore production. Four popular local varieties viz., JS-335, PK-1029, MACS- 124 and LSB were selected and evaluated for nitrogen fixation responsiveness. Among the four varieties tested, JS-335 showed better response to commercial Bradyrhizobium (CRIDA Soya 809). Further, JS-335 variety was tested against the rhizobial isolates for effective root nodulation, of which SBR-8 resulted in effective symbiosis indicating, more nodule number, nodule and plant dry weight. Total Nitrogen content of plant and soil were also increased along with microbial population in the rhizosphere of soybean crop. From the findings of the research, it is concluded that, SBR-8 rhizobial isolate is better suited for the development of a commercial biofertilizers for JS-335 variety.