BIODEGRADATION OF COMPLEX HYDROCARBON COMPOUNDS BY SOIL ISOLATES OF DEHRADUN REGION, INDIAKUNAL MADHAV, NEHA TANWAR, NUPUR GUSAIN, LATIKA SHARMA AND ADITYA CHAUDHARYAbstract PAH like naphthalene are so toxic there is interest in understanding the physicochemical processes and microbial degradation reactions that affect the mobility and fate of these compounds in groundwater and soil sediment system. The present work focuses on Biodegradation of Petroleum Hydrocarbons by bacterial soil isolates of soil samples were collected in sterile containers from petrol pumps. The soil sample from selected sites were diluted and plated on sterile NAM plates. Colonies of Bacillus spp., Klebsiella spp., Paracoccus spp. and Paenibacillus spp. were obtained. The isolates obtained were then tested against Petrol, Diesel, Lubricating oil and Naphthalene which were incorporated in Basal Salt Media (broth and agar) and were incubated at 30oC for five days. Readings from the broth culture was recorded every 24 hours at 595 nm for five days and the results were depicted graphically. The results obtained were compared with hydrocarbon utilizing Pseudomonas aeruginosa MTCC 2474 strain.
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