Ecology, Environment and Conservation Paper

EVALUATION OF DIFFERENT ORGANIC SUBSTRATES FOR MASS MULTIPLICATION OF PSEUDOMONAS FLUORESCENS

Prashant Kumar Dewangan, Niranjan Khare, Bhumika Koma, Sanghmitra Baghel and H.K. Singh

Abstract

The present investigation was carried out to screen different organic substrate for simple, cheap and effective method of mass multiplication Pseudomonas fluorescens. Different organic substrates were sterilizated by different methods such as hot water, steam and chemical treatment at different incubation temperatures. 200 g of each substrate was filled in polythene bag and 48 h KMB slant culture of P. fluorescens cell suspension of concentration of 108 cfu/mL was pipetted into each bag. The observations were recorded on 7th, 14th, 21st, 28th, and 35 th days after inoculation (DAI). One gram of substrate from each bag was used for assessment of difference in growth of P. fluorescens and data regarding number of colony forming units (cfu) of bioagent per gram of each substrate was recorded. At 35 DAI, significantly higher population of P. fluorescens was observed in FYM followed by vermi compost and gram straw at temperatures (20-25oC) and (35-40oC) irrespective of the three sterilization methods. It was also observed that overall growth of the P. Fluorescens isolate was maximum in steam based sterlization followed by hot water and least in chemical method of sterilization at both the temperatures (20-25oC) and (35-40oC).