OPTIMIZATION OF PROCESS PARAMETERS INFLUENCING DECOLOURIZATION OF NAVY BLUE-HER USING SIMPLEX CENTROID MIXTURE DESIGNG. PREETHI, S. SRIDHAR AND J. HELAN CHANDRAAbstract Dyes as coloring agent used extensively in the textile industry are difficult to degrade by most conventional methods. The present work compares the ability of individual and mixture of microorganisms on decolorizing textile dye Navy Blue-HER. Pseudomonas putida, Bacillus subtilis, Escherichia coli and bacterial consortium were used to decolorize the Navy Blue -HER. The effect of physiochemical parameters such as pH, temperature, dye concentration and incubation time were studied using One Factor at a time (OFAT) for culture supernatant of individual and consortium towards dye decolorization process. For Pseudomonas putida, the optimum pH, temperature and dye concentration was found to be pH 8, 40°C and 150 mg/L respectively. For Bacillus subtilis, the optimum pH, temperature and dye concentration were found to be pH 8, 40°C and 100 mg/L respectively. For E. coli, the optimum pH, temperature and dye concentration were found to be pH 7, 50°C and 200 mg/L respectively. Simplex centroid method was adopted to identify the influence of individual variable. Among the three organisms, Bacillus subtilis were found to be the most influencing organism. For bacterial consortium, the optimum conditions were found to be pH 7, temperature 50°C and dye concentration 100 mg/L. The optimal incubation time for single pure culture supernatant such as Bacillus Subtilis, Escherichia coli and culture supernatant of bacterial consortium were found to be four hours, while for Pseudomonas putida is five hours respectively.
Enter your contact information below to receive full paper.
|