Pollution Research Paper

Vol 34, Issue 3, 2015; Page No.(629-634 )

EFFECT OF DIFFERENT CULTURE CONDITIONS ON THE PRODUCTION OF LACCASE FROM BASIDIOMYCETES FUNGAL ISOLATE PLEUROTUS OSTREATUS VPM -1 UNDER SOLID STATE FERMENTATION

R.KOWSALYA, S. SANKARANARAYANAN, A. UMA AND SWARANJIT SINGH CAMEOTRA

Abstract

The production of laccase by Pleurotus ostreatus was studied on solid state fermentation. Culture parameters such as type and concentration of substrate, pH, moisture, and temperature were optimized in solid state fermentation .Substrates such as coconut coir, paddy straw, rice bran, wheat bran, and sugarcane bagasse were tested to assess their suitability to produce maximum yield of laccase. pH ranges tested varied from 4 to 8. Moisture (%) tested varied from 40% to 80%. Temperatures at which the laccase yield was tested were 30°C to 70°C. The maximum laccase yield of 270.4 U/mL was obtained in rice bran as substrate with 70% moisture content, pH 6.0, and temperature 40°C. Pleurotus ostreatus is a potential producer of laccase enzyme using solid state fermentation and can be exploited for further biotechnological applications. This optimized methodology in this study offers scope for economic utilization and value addition of agro – residues.

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