Ecology, Environment and Conservation Paper

IDENTIFICATION OF TRITICUM AESTIVUM L. GENOTYPE BY ALLELIC VERSIONS OF WAXY-GENES AND HMW GLUTENIN SUBUNITS

Vafin R.R., Rzhanova I.V., Askhadullin D-l.F., Askhadullin D-r.F., Vasilova N.Z., Tagirov M.Sh., Tyulkin S.V., Abdulina I.R. and Shagalieva G.I.

Abstract

This paper presents molecular genetic assessment of 87 spring wheat samples of local (mostly) and foreign selection from the collection of Tatar Agricultural Research Institute of the RAS in order to identify genotypes of Triticum aestivum L. by allelic versions of Waxy-genes and HMW glutenin subunits, along with identification of the most promising genotypes bearing selectionally useful alleles associated with good milling, baking and technological properties of the grain. The results of identification by allelic variants of Waxy-genes have revealed 4 most promising genotypes (4.6%) with the Wx-A1a/B1b/D1a combination which can serve as parent material for following selection of breeds of spring wheat containing amylopectin starch with null Wx-B1b-allele in its genotype. Analysis of distribution of genotypes under study by aggregate combination of Glu-A1/D1-locuses of HMW glutenin subunits revealed prevalence of useful combinations of subunits Ax2*/5+10 with good milling and baking properties of grain (37 samples, 42.5%).