ANTIBODY RESPONSE TO TUMOR SPECIFIC ANTIGEN AND ITS ASSOCIATION WITH ANTIOXIDANT ENZYMES IN DIFFERENT HISTOPATHOLOGICAL STAGES OF EXPERIMENTAL MURINE SKIN CARCINOMASOLAI SOPHIA AND MUDIGERE M. RAMESHAAbstract Present study was carried out to find the correlation between antioxidant enzyme status and antibody response to tumor specific antigen in serum of the skin carcinoma mice. Skin carcinoma was induced using 7, 12-Dimethylbenz [a] anthracene; the mice at different stages of tumor development were diagnosed using histopathological analysis and selected for the study. Reduced glutathione and glutathione–S–transferase enzymes were measured from the crude enzyme fraction from freshly dissected liver sample. Tumor specific antigen was isolated using SDS-PAGE and autoantibody in the serum was meseared using ELISA. There was significant increase in the enzyme activity during the early stage of tumor progress and the level of auto antibodies to the tumor specific antigen was significantly high during the early progressive stages when compared with later stages. The increased activities of antioxidant enzymes may be compensatory regulation in response to increased oxidative stress during the early and progressive stages. Hence treatment with antioxidants in the initial stages of the disease may be useful as secondary therapy to prevent the oxidative damage and to improve the immune function. The results also insist the necessity to find out the antioxidant status of each patient for therapeutic co-administration of antioxidants along with chemotherapy.
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