ISOLATION, CHARACTERIZATION AND IDENTIFICATION OF ROUNDUP DEGRADING BACTERIA FROM THE AGRICULTURAL AREA OF THAILANDPANNAPA POWTHONG, BAJAREE JANTRAPANUKORN AND PATTRA SUNTORNTHITICHAROENAbstract Glyphosate is a base formulation ingredient in Roundup which is used as herbicide in the control of grasses and weed plants. Since its property is non-selective herbicide and extensive consumes by human, therefore bioremediation is a useful method to decontaminate glyphosate contaminated soils. In this experiment, the potential for biodegradation of glyphosate through enrichment and isolation of glyphosate biodegraders from soil and water in agricultural area was done. Microorganisms grew in minimal media with glyphosate as the only carbon source. Five from 19 bacteria isolated were selected by increasing concentrations of glyphosate in Roundup. The glyphosate degradation was quantified by the HPLC places degrading bacteria namely WPTR1.1.2-34%, WPTR5.41.1, 2, 3-35%, WPTR28.35.1-47.5%, WPTR 28.41.7, 8- 49.3%, and WPTR28.41.9-52.17% respectively in 7 days. Further, the optimal of temperature, pH, and salinity of the isolate has also been investigated and revealed that an optimum temperature at 37 45oC and was able to growth at a wide range of NaCl and pH. These promising glyphosate degrading isolates were tentatively identified biochemical and MALDI-TOF MS characterization as Klebsiella pneumoniae. It was indicated that K. pneumonia which isolated in this study could be useful in glyphosate biodegradation in agricultural area.
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