Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 18, Issue 3, 2016; Page No.(735-742)

ISOLATION AND PRODUCTION OF BACTERIAL ACIDOTHERMOPHILIC AND ORGANIC SOLVENT TOLERANT XYLANASE

RADHIKA KASHYAP , MONIKA AND SANDEEP TRIPATHI

Abstract

Lignocellulosic biomass has the potential to replace fossil fuels as the ultimate source of energy which in turn will tone down the disastrous effect of green house gases and global warming. Lignocellulosic biomass especially non-edible plant material can provide fermentable sugars which further can be converted into various energy rich compounds. For many years xylanase is being used actively for the conversion of xylan, the most common hemicellulosic component of biomass into various bioproducts and fuels. However, xylanase which can give cost competitive sugars remains a challenge. Present study involved screening of a xylanase producing bacterial isolate from hydrocarbon contaminated soil. Based on 16S rRNA gene analysis, the bacterial isolate was identified as Bacillus sp. R5-93. Upon characterization, crude xylanase by Bacillus sp. R5-93 was found to be cellulase free, acidothermophilic and organic solvent tolerant. Xylanase showed optimum activity at 50 oC and pH 5. Tolerance to organic solvents such as n-hexane, acetone, methanol, glycerol, isoamyl alcohol, diethyl ether and petroleum ether was also observed. Besides that, successful production of xylanase using agro-residues was done under submerged fermentation. These features make it a potential candidate for economic applicability in food, feed and biofuel industry.

Enter your contact information below to receive full paper.
Your Name :
Email:
Phone:
City:
Cost of Full Paper: Rs.75 for Indian Nationals or $20 (USD) for international subscribers.
By clicking on Request Paper you Agree to pay the above mentioned cost per paper.
  
Journal Issues
Vol 19, Issue 2, 2017
Vol 19, Issue 1, 2017
Vol 18, Issue 4, 2016
Vol 18, Issue 3, 2016
Vol 18, Issue 2, 2016
Vol 18, Issue 1, 2016
Vol 17, Issue 4, 2015
Vol 17, Issue 3, 2015
Vol. 17 Special Issue 2015
Vol 17, Issue 2, 2015
Vol 17, Issue 1, 2015
Vol 16, Issue 4, 2014
Vol 16, Issue 3, 2014
Vol 16, Issue 2, 2014
Vol 16, Issue 1, 2014
Vol 15, Issue 4, 2013
Vol 15, Issue 3, 2013
Vol 15, Issue 2, 2013
Vol 15 Issue 1, 2013
Vol 14, Issue 4, 2012
Vol 14, Issue 3, 2012
Vol 14, Issue 2, 2012
Vol.14, Issue 1, 2012
Vol.13, Issue 4, 2011
Vol.13, Issue 3, 2011
Vol.13, Issue 2, 2011
Vol.13, Issue 1, 2011
Vol.12, Issue 4, 2010
Vol.12, Issue 3, 2010
Vol.12, Issue 2, 2010
Vol.12, Issue 1, 2010
Vol 11, Issue 4, 2009
Vol 11, Issue 3, 2009
Vol 11, Issue 2, 2009
Vol 11, Issue 1, 2009
Vol 10, Issue 4, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 2, 2008
Vol 10, Issue 1, 2008
Vol 9, Issue 4, 2007
Vol 9, Issue 3, 2007
Vol 9, Issue 2, 2007
Vol 9, Issue 1, 2007
Vol 8, Issue 4, 2006
Vol 8, Issue 3, 2006
Vol 08, Issue 2, 2006
Vol 08, Issue 1, 2006
Vol 7 Issue 4, 2005
Vol 07, Issue 3, 2005
Vol 07, Issue 2, 2005
Vol 6 Issue 4, 2004
Vol 6 Issue 3, 2004
Vol 6 Issue 2, 2004
Vol 6 Issue 1, 2004
Vol 5 Issue 3, 2003
Vol 5 Issue 2, 2003
Vol 04, Issue 4, 2002
Vol 04, Issue 1, 2002
Vol 3 Issue 1-2, 2001
Vol 1 Issue 1-2, 2000
Vol 1 Issue 3-4, 1999
Vol 1 Issue 1-2, 1999
Looking for Past Issues? Click here to get them!!