Ecology, Environment and Conservation Paper

ECO-FRIENDLY MANAGEMENT OF MAGNAPORTHE GRISEA CAUSING BLAST IN BASMATI RICE

Vipin Kumar, Prashant Mishra, Ramji Singh, Kamal Khilari, Gopal Singh, S. K. Sachan, Mehi Lal and Gireesh Chand

Abstract

Management of Pyricularia oryzae using eco-friendly approaches is an important option to reduce the chemical toxicity in plant produces. In present study we extracted and tested the essential oils from Coriander sativum (Coriander), Azadirachta indica (Neem) and Syzygium aromaticum (Clove) for their effect against Pyricularia oryzae in Basmati rice. These oils were evaluated for their antifungal activity against P. oryzae in vitro by poison food technique. Isolated and purified culture of P. oryzae was maintained on potato dextrose agar (PDA) medium for further experiments. The pure culture of P. oryzae was tested against different concentration (300, 500 and 1000ppm) of essential oils on PDA medium at 72 hrs, 96 hrs and 120 hrs intervals. Maximum inhibition of mycelial growth was observed in clove oil which showed 100% of inhibition at 300, 500 and 1000ppm. Next effective oil was observed neem oil followed by coriander oil. This indicated that clove oil is most effective against P. oryzae pathogen. Neem and coriander oil is less effective. The results of the current study indicated that each of the three essential oils tested, reduced the radial growth of P. oryzae.