Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol.12, Issue 3, 2010; Page No.(677-680)

ISOLATION OF PCR COMPATIBLE DNA FROM SWERTIA CHIRAYITA

VIKAS SHARMA, NIDHI SRIVASTAVA, BARKHA KAMAL, A.K. DOBRIYAL AND VIKASH S. JADON

Abstract

Swertia chirayita is a medicinal plant indigenous to temperate Himalaya. This plant contains high amounts of secondary metabolites that interfere with DNA isolation. Good quality DNA is always a prerequisite in all molecular biology experiments especially PCR. Several protocols described for plant DNA isolation fail to produce good quality DNA from medicinal herbs and aromatic plants. Our modified DNA isolation method yields good-quality, high-molecular weight DNA that is free of contaminants and colored pigments and is suitable for PCR amplification.

Enter your contact information below to receive full paper.
Your Name :
Email:
Phone:
City:
Cost of Full Paper: Rs.150 for Indian Nationals or $20 (USD) for international subscribers.
By clicking on Request Paper you Agree to pay the above mentioned cost per paper.