Ecology, Environment and Conservation Paper

Vol 23, Issue 1, 2017; Page No.(323-330)

MUTAGENESIS OF PACHYSOLEN TANNOPHILUS AND ITS PROTOPLAST FUSION WITH SACCHAROMYCES CEREVISIAE FOR ETHANOL PRODUCTION

Sushma Gurumayum*, Krishan Lal Kalra and Gurvinder Singh Kocher

Abstract

The study deals with mutagenesis of P. tannophilus MTCC1077 and protoplast fusion between S. cerevisiae G and P. tannophilus MTCC1077 to obtain variants and fusants capable of ethanol production from xylose. A total of 103 and 102 ethanol negative variants were selected from mutagenesis with UV and EMS respectively. The parent P. tannophilus MTCC1077 gave maximum ethanol yield of 0.339g/g with a corresponding fermentation efficiency of 66.47% from 20g/l xylose when fermentation was carried out for 120hr at 30ÂșC at a pH of 5.0 with 6% v/v inoculum and aeration of 100rpm. Two ethanol negative mutants UVmut 62 and UVmut 41 gave ethanol yields 0.347 g/g and 0.350g/g respectively. Three ethanol negative mutants EMSmut14, EMSmut39 and EMSmut26 gave ethanol yield of 0.346g/g 0.350g/g, 0.364g/g respectively. S.cerevisiae G gave ethanol yield of 0.430g/g with a fermentation efficiency of 84.21%. Protoplast fusion between S. cerevisiae G and P. tannophilus MTCC1077 yielded two stable fusants FUS1 and FUS2 which gave yield of 0.244g/g and 0.273g/g respectively from a mixture of glucose and xylose after 72hrs of fermentation. Scanning electron microscopy (SEM) study of parent strains and fusants revealed differences in the cell surface and also in budding pattern as compared to that seen with either of the individual parent strains. DNA contents of the two fusants were larger than that of any single parental strains, suggesting that these fusants have inherited genetic material from both parental strains.

Enter your contact information below to receive full paper.
Your Name :
Email:
Phone:
City:
Cost of Full Paper: Rs.75 for Indian Nationals or $20 (USD) for international subscribers.
By clicking on Request Paper you Agree to pay the above mentioned cost per paper.
  
Journal Issues
Vol 23, Nov. Suppl. Issue 2017
Vol 23, Sept. Suppl. Issue 2017
Vol 23, Issue 2, 2017
Vol 23, Issue 3, 2017
Vol 23, Issue 1, 2017
Vol 23, Feb 2017 Suppl. Issue
Vol 22, Dec 2016 Suppl. Issue
Vol 22, Issue 4, 2016
Vol 22, Sept. Suppl. Issue , 2016
Vol 22, Issue 3, 2016
Vol. 22, June Suppl. Issue 2016
Vol 22, Issue 2, 2016
Vol. 22, April Suppl. Issue 2016
Vol 22, Issue 1, 2016
Vol 21, Issue 4, 2015
Vol. 21 Dec. 2015 Suppl. Issue
Vol. 21 Nov. 2015 Suppl. Issue
Vol 21, Issue 3, 2015
Vol 21, Issue 2, 2015
Vol. 21 Suppl.Issue August 2015
Vol 21.Suppl.Issue June 2015
Vol 21, Issue 1, 2015
Supplement Issue, Dec. 2014
Special Issue-2014
Vol 20, Issue 4, 2014
Vol 20, Issue 3, 2014
Vol 20, Issue 2, 2014
Vol. 20 Issue 01, 2014
Vol. 19 Issue 04, 2013
Vol. 19 Issue 03, 2013
Vol. 19, Issue 02, 2013
Vol. 19, Issue 01, 2013
Vol.18, Issue 04, 2012
Vol.18, Issue 3, 2012
Vol.18, Issue 2, 2012
Vol.18, Issue 1, 2012
Vol.17, Issue 4, 2011
Vol.17, Issue 3, 2011
Vol.17, Issue 2, 2011
Vol.17, Issue 1, 2011
Vol.16, Issue 4, 2010
Vol.16, Issue 3, 2010
Vol.16, Issue 2, 2010
Vol.16, Issue 1, 2010
Vol.15, Issue 04, 2009
Vol.15, Issue 03, 2009
Vol.15, Issue 02, 2009
Vol.15, Issue 1, 2009
Vol.14, Issue 04, 2008
Vol.14, Issue 2-3, 2008
Vol.14, Issue 2-3, 2008
Vol.14, Issue 1, 2008
Vol.14, Issue 2-3, 2008
Vol.13, Issue 04, 2007
Vol.13, Issue 2, 2007
Vol.13, Issue 1, 2007
Vol.12, Issue 4, 2006
Vol.12, Issue 3, 2006
Vol.12, Issue 2, 2006
Vol.12, Issue 1, 2006
Vol.12, Issue 01, 2006
Vol.11, Issue 3,4, 2005
Vol.11, Issue 2, 2005
Vol.11, Issue 1, 2005
Vol.10, Issue 04, 2004
Vol.10, Issue 03, 2004
Vol.10, Issue 02, 2004
Vol.10, Issue 01, 2004
Vol.09, Issue 04, 2003
Vol.09, Issue 03, 2003
Vol.09, Issue 02, 2003
Vol.08, Issue 04, 2002
Vol.08, Issue 03, 2002
Vol.08, Issue 01, 2002
Vol.07, Issue 04, 2001
Vol.07, Issue 03, 2001
Vol.07, Issue 02, 2001
Vol.07, Issue 01, 2001
Vol.06, Issue 04, 2000
Vol.06, Issue 03, 2000
Vol.06, Issue 02, 2000
Vol.06, Issue 01, 2000
Vol.05, Issue 04, 1999
Vol.05, Issue 03, 1999
Vol.05, Issue 02, 1999
Vol.05, Issue 01, 1999
Vol.04, Issue 1,2, 1998
Vol.03, Issue 3,4, 1997
Vol.03, Issue 01, 1997
Vol.02, Issue 1,2, 1996
Vol.01, Issue 14, 1995
Looking for Past Issues? Click here to get them!!