Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 19, Issue 2, 2017; Page No.(397-403)

AG-NANOPARTICLES BASED ON POLYSACCHARIDE ISOLATED FROM THE LEAVES OF XYLOCARPUS GRANATUM AND THEIR STUDY ON MICROBES AND COMPARISON WITH CIPRAN 500

GAJENDRA NATH MAITY AND SOUMITRA MONDAL

Abstract

In modern research, the field of nanotechnology/nanoscience is one of the most interesting areas for chemists, biologists/microbiologists for their commercial demand and it emerges rapidly owing to its wide application in chemistry, electronics ecology, and medicine as well as in biological fields. Economical preparations of silver (Ag) nanoparticles (NPs) using green synthesis path having biological entities are gradually increasing. Here an attempt was made to green synthesis silver nanoparticles (SNPs) using polysaccharide extracted from the leaves of Xylocarpus granatum. Polysaccharide-based metallic nanoparticles have an increasing commercial demand because of their wide applicability in various areas such as electronics, chemistry, energy, cosmetics, and medicine. In recent times, Au- or Ag-nanoparticles based on synthetic polysaccharide or plant or animal polysaccharide is unique. Thus, nanoparticles synthesized from them may show some different activity. In the present study, SNPs were prepared by using polysaccharide isolated from the leaves of Xylocarpus granatum and characterized by using UV-VIS spectrum band at 426 nm. Here, the polysaccharide acts as reducing and also stabilizing agent. The synthesized SNPs were found to be very effective as antimicrobial agents against some important human pathogens such as Escherichia coli ATCC 25922, Salmonella typhimurium, Salmonella typhi MTCC 734, which are affecting and causing serious problems to human beings. These SNPs also have sporocidal activity. These SNPs can also reduce the microbial load by destroying the microorganisms present in sewage water. Effectiveness of these nanoparticles was compared with Cipran-500.

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