Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 19, Issue 2, 2017; Page No.(412-418)

EFFECT OF PHYSICO- CHEMICAL FACTORS ON THE PRODUCTION OF CONJUGATED LINOLEIC ACID BY LACTOBACILLUS PLANTARUM

PALEMPALLI UMA MAHESWARI DEVI AND H.K. RASHMI

Abstract

The present study was designed to study the efficiency of Probiotic bacteria Lactobacillus plantarum for the production of conjugated linoleic acid isomers from linoleic acid. The optimal pH and temperature for the production of LP-CLA by the prospective culture was determined. The temperature at 40ÂșC and pH of 6.5 served as the best for the optimal conversion of LA to CLA by Lactobacillus plantarum with a yield of 37.5μg/mL. The effect of incubation time on LP-CLA production and weight of the biomass was studied at different time points. The LP-CLA production was in correlation with the weight of the biomass, and the maximum yield was found during the stationary phase. Influence of LA concentration on the growth of Lactobacillus plantarum and LP-CLA was studied in the presence of different concentrations of LA. The initial concentration of 100μg/mL served ideal for the production of LP-CLA and growth of Lactobacillus plantarum. Further increase in LA concentration decreased the conversion rate of LP-CLA production. The production rate of LP-CLA was increased from 37.5 μg/mL to 76.6 μg/mL by supplementing the MRS medium with mixture of glucose and yeast extract at the ratio 1:2.

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