Ecology, Environment and Conservation Paper

Vol 23, Issue 2, 2017; Page No.(1043-1055)

COMPARISON OF IN VITRO PLANTLET REGENERATION APTITUDE OF THREE CITRUS SPECIES FROM CULTURED NODAL SEGMENTS VIA ORGANOGENIC MODE

Megha Vibhute, M.K. Tripathi, R. Tiwari R.P. Patel, B.S. Baghel and Ashok Ahuja

Abstract

Nodal segments excised from in vitro germinated seedlings of three citrus species viz: Citrus aurantiafolia, Citrus reticulata and Citrus sinensis were cultured on different fortifications of basal MS medium for multiple shoot proliferation. In vitro morphogenesis leading to plantlet regeneration varied considerably among species and with medium composition. In the present study, plantlets were regenerated directly via auxiliary bud proliferation as well as from callus culture. Induction medium MS5N.5B/MS5N.Kn (MS + 5.0 mgL-1 NAA + 0.5 mgL-1 BA/Kn) induced callusing in higher frequencies. Culture medium MS.1Td.5N/MS.5B.5N (MS + 0.1 mgL-1 TDZ/0.5 BA + 0.5 mgL-1 NAA) enhanced shoot proliferating efficiency. Shoot (s) per explants in higher number (s) of higher length were documented on medium MS.1Td or MS.2Td (MS + 0.1/0.2 mg L-1 TDZ). Higher root proliferating efficiency was recorded on rooting medium MS.5IB (MS + 0.5 mg L-1 IBA). Roots in higher frequency were observed on culture medium MS2IB.5Kn (MS + 2.0 mg L-1 IBA + 0.5 mg L-1 Kn), while culture media MS.5IB.5B (MS + 0.5 mg L-1 IBA + 0. 5 mg L-1 BAP) enhanced mean root length. In terms of intraspecific in vitro response, in general, Acid lime followed by Mandarin and Sweet orange responded decisively for the most of the culture phases. The in vitro raised plantlets were successfully acclimatized and transferred to the field.

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