Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 19, Issue 3, 2017; Page No.(491-495)

MYCOBACTERIUM TUBERCULOSIS DETECTION FROM DUST AND WATER SAMPLE IN HOUSE OF BTA (+) LUNG TUBERCULOSIS PATIENT THROUGH POLYMERASE CHAIN REACTION METHOD IN MALANG

DWI YUNI NUR HIDAYATI, NGAKAN PUTU PARSAMA PUTRA, HARUN AL RASYID, FATIMA K. MEGAWATI AND WIRA NIRWANA

Abstract

Tuberculosis is one of health problems in the worldwide and one of the endemic countries is Indonesia. The prevalence of tuberculosis in East Java is considered as one of the highest in Indonesia, for instance, the prevalence in Malang is 0.4% with 60% of it are detected as BTA (+). This study was conducted to investigate M. tuberculosis bacteria in dust and water sample that are found surrounding the home of tuberculosis patients in Sukun district in Malang. Samples were analyzed using Polymerase Chain Reaction (PCR) with Pt 3 and Pt 6 primers. Pt 3 and Pt 6 located in the 188 bp as well as the primary Tr 8 and Tr 9 is located at 157 bp. Bacteria were cultured in Middle Brook medium and Lowenstein- Jensen. There are 5 samples (27.8%) detected as positive and the rest of it or 13 samples are negative. Positive samples PCR band show similarities with band 100-200 bp. Furthermore, there are 6 water samples (33%) that detected as positive with the appearance of 188 bp bands. These results indicate the transmission risk in Sukun area so there will be more studies required.

Enter your contact information below to receive full paper.
Your Name :
Email:
Phone:
City:
Cost of Full Paper: Rs.75 for Indian Nationals or $20 (USD) for international subscribers.
By clicking on Request Paper you Agree to pay the above mentioned cost per paper.
  
Journal Issues
Vol 19, Issue 3, 2017
Vol 19, Issue 2, 2017
Vol 19, Issue 1, 2017
Vol 18, Issue 4, 2016
Vol 18, Issue 3, 2016
Vol 18, Issue 2, 2016
Vol 18, Issue 1, 2016
Vol 17, Issue 4, 2015
Vol 17, Issue 3, 2015
Vol. 17 Special Issue 2015
Vol 17, Issue 2, 2015
Vol 17, Issue 1, 2015
Vol 16, Issue 4, 2014
Vol 16, Issue 3, 2014
Vol 16, Issue 2, 2014
Vol 16, Issue 1, 2014
Vol 15, Issue 4, 2013
Vol 15, Issue 3, 2013
Vol 15, Issue 2, 2013
Vol 15 Issue 1, 2013
Vol 14, Issue 4, 2012
Vol 14, Issue 3, 2012
Vol 14, Issue 2, 2012
Vol.14, Issue 1, 2012
Vol.13, Issue 4, 2011
Vol.13, Issue 3, 2011
Vol.13, Issue 2, 2011
Vol.13, Issue 1, 2011
Vol.12, Issue 4, 2010
Vol.12, Issue 3, 2010
Vol.12, Issue 2, 2010
Vol.12, Issue 1, 2010
Vol 11, Issue 4, 2009
Vol 11, Issue 3, 2009
Vol 11, Issue 2, 2009
Vol 11, Issue 1, 2009
Vol 10, Issue 4, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 3, 2008
Vol 10, Issue 2, 2008
Vol 10, Issue 1, 2008
Vol 9, Issue 4, 2007
Vol 9, Issue 3, 2007
Vol 9, Issue 2, 2007
Vol 9, Issue 1, 2007
Vol 8, Issue 4, 2006
Vol 8, Issue 3, 2006
Vol 08, Issue 2, 2006
Vol 08, Issue 1, 2006
Vol 7 Issue 4, 2005
Vol 07, Issue 3, 2005
Vol 07, Issue 2, 2005
Vol 6 Issue 4, 2004
Vol 6 Issue 3, 2004
Vol 6 Issue 2, 2004
Vol 6 Issue 1, 2004
Vol 5 Issue 3, 2003
Vol 5 Issue 2, 2003
Vol 04, Issue 4, 2002
Vol 04, Issue 1, 2002
Vol 3 Issue 1-2, 2001
Vol 1 Issue 1-2, 2000
Vol 1 Issue 3-4, 1999
Vol 1 Issue 1-2, 1999
Looking for Past Issues? Click here to get them!!